Integrating a lock-in amplifier into an SRS microscopy experiment requires careful consideration of several factors. Firstly, the choice of reference signal frequency is critical, as it should match the frequency of the desired Raman scattering signal. This ensures optimal phase synchronization and maximizes the extraction of the desired signal. Additionally, the lock-in amplifier should be properly calibrated to account for any phase shifts or delays introduced by the experimental setup. This calibration process involves adjusting the phase and gain settings of the SRS lock in amplifier to achieve accurate and reliable signal extraction.
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